Journal article
Structural insights into the multifunctionality of rabies virus P3 protein
A Sethi, SM Rawlinson, A Dubey, CS Ang, YH Choi, F Yan, K Okada, AM Rozario, AM Brice, N Ito, NA Williamson, DM Hatters, TDM Bell, H Arthanari, GW Moseley, PR Gooley
Proceedings of the National Academy of Sciences of the United States of America | Published : 2023
Abstract
Viruses form extensive interfaces with host proteins to modulate the biology of the infected cell, frequently via multifunctional viral proteins. These proteins are conventionally considered as assemblies of independent functional modules, where the presence or absence of modules determines the overall composite phenotype. However, this model cannot account for functions observed in specific viral proteins. For example, rabies virus (RABV) P3 protein is a truncated form of the pathogenicity factor P protein, but displays a unique phenotype with functions not seen in longer isoforms, indicating that changes beyond the simple complement of functional modules define the functions of P3. Here, w..
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Grants
Awarded by Australian Research Council
Funding Acknowledgements
& nbsp;We acknowledge Cassandra David for assistance with tissue culture and Kirsten Elgass (Monash Micro Imaging, Monash University; currently at Carl Zeiss Microscopy in Jena, Germany) for assistance with the superresolution microscope. A.M.R. acknowledges the Bendigo Tertiary Education Anniversary Foundation for supporting the Holsworth Biomedical Research Initiative. This work was partly supported by National Health and Medical Research Council grants 1125704 to G.W.M. and P.R.G.; 1160838 and 1079211 to G.W.M.; Australian Research Council grants DP210100998 to P.R.G., G.W.M., and H.A.; DP170104477 to T.D.M.B.; DP150102569 to G.W.M.; and a Grimwade Fellowship provided by the Meigunyah Fund to G.W.M. We acknowledge the use of the biological SAXS beamline of the Australian Synchrotron, part of the Australian Nuclear Science and Technology Organisation and the mass spectrometry and NMR facilities at the University of Melbourne and Harvard University and the & nbsp;facilities and technical assistance of the Monash Micro Imaging facility (Monash University).